Journal: iScience
Article Title: A mathematical kinetic model of memory in Bacillus subtilis spore germination
doi: 10.1016/j.isci.2026.115864
Figure Lengend Snippet: A double pulse germination experiment with B. subtilis wild-type spores and spores of an isogenic strain that overexpresses the GerA GR (A) Wild-type B. subtilis spores (PS533) were given two 2-min 10 mM L-valine (L-val) germinant pulses separated by 30 min. (B) Spores of B. subtilis PS3476 (GerA GR over-expressed ∼8-fold) were given two 2-min 10 mM L-valine germinant pulses separated by 30 min. Gray bars above the horizontal axis indicate pulse durations. (C) Simulated percentage germination of WT spores in a two pulse germination experiment. The estimated distribution mean is 1,118 and the standard deviation is 1,064 with a is 1.10476 [0.989585, 1.23334] and b is 1012.19 [881.615, 1162.1]. At zero time R a = 0 copies/spore; C c = 6,500 copies/spore. C a = C o = 0 copies/spore; k 1 = 350e−5; k -1 = 100e−3; k 2 = 25e−7; k 3 = 60e−3; k 4 = 195e2; k 5 = 305e−2; n = 3; θ = 20. (D) Simulated percentage germination of overexpressed GerA GR spores in a two pulse germination experiment. The estimated distribution mean is 3,367 and the standard deviation is 399 with a is 71.2149 [62.9306, 80.5898] and b is 47.2855 [41.7667, 53.5336]. At zero time R a = 0 copies/spore; C c = 6,500 copies/spore. C a = C o = 0 copies/spore; k 1 = 350e−5; k -1 = 100e−3; k 2 = 25e−7; k 3 = 60e−3; k 4 = 195e2; k 5 = 305e−2; n = 3; θ = 20.
Article Snippet: L-Valine (≥99%) , Thermo Scientific Chemicals (Thermo Fisher Scientific) , Cat A18894.06.
Techniques: Standard Deviation